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Panleukopenia serum

Efficacy of feline serum transfusion for prevention or

In one of your online resources/publications regarding feline panleukopenia virus, it states that, Passive protection from serum transfusion has been shown in puppies exposed to canine parvovirus FELINE PARVO ---- PANLEUKOPENIA, FELINE DISTEMPER, CAT FEVER 12 HR NDV INDUCED SERUM IN THE FELINE Give 1/2 cc of NDV vaccine IV to cat. Wait 12 hours. Anesthetize. Shave neck and take blood from jugular vein. Clot and remove serum. Place in clean 10 cc sterile bottles and store in refrigerator till needed

Bi curs an5 panleucopenia felina

Feline panleukopenia is a parvoviral infectious disease of kittens typically characterized by depression, anorexia, high fever, vomiting, diarrhea, and consequent severe dehydration. Adult cats are much less often affected. Diagnosis is usually based on clinical signs, severe neutropenia and lymphopenia, and fecal viral antigen or PCR testing We present a successful approach for treating feline panleukopenia, a fatal and highly contagious disease. It involves recognizing the disease early though the uses of parvo test kits or low neutrophil count; applying the drug Neupogen (filgrastim); and providing antibiotics, SC fluids and other supportive therapies Feline panleukopenia virus Consistent with this hypothesis is the finding that antibodies to a CPV-2-like virus were first detected in serum from European dogs taken in the early to mid 1970s, but only in 1978 did explosive spread of the virus occur. Also of interest for this hypothesis is the fact that a variant of CPV-2 called CPV-2a. Feline panleukopenia virus (FPV) is the prototype of closely related parvoviruses isolated from dogs, mink, raccoons, raccoon dogs, foxes and other canids (Parrish, 1990). They were initially named after the hosts from which they had been isolated

(PDF) Vaccination against Feline Panleukopenia

Feline infectious enteritis (FIE) is a disease caused by infection with feline parvovirus (FPV), also known as feline panleukopenia virus. It is sometimes referred to as panleukopenia virus because one of the results of infection is the development of a low white blood cell count (which is what panleucopenia means) Feline panleukopenia virus (FPV) is a severe, highly contagious parvoviral infection of cats. Panleukopenia is now a relatively rare disease in pet cats because of highly effective vaccines. Occasional infections are seen in unvaccinated kittens, especially those from shelters, farms, and urban stray populations Panleukopenia or FPV is a highly contagious virus that often affects kittens under the age of one. It is a very resistant virus that can be fatal (1, 2, 3). This virus is most commonly seen in kittens under the age of one, once maternal antibodies are gone. It more commonly however affects those between the age of 3-5 months (4) To evaluate the accuracy of hemagglutination inhibition (HI) test as the index of feline panleukopenia virus (FPV)-protective ability, sera from 153 FPV-vaccinated cats aged ≥7 months with HI titer of <1:10-1:40, were examined for serum neutralizing (SN) antibody. SN antibody was detected (≥1:10) in 33 (62.3%) of 53 HI antibody-negative cats, and ranged <1:10-1:160 Feline Panleukopenia Vaccine shall be prepared from virus-bearing cell culture fluids. Only Master Seed Virus which has been established as pure, safe, and immunogenic shall be used for preparing the production seed virus for vaccine production

  1. panleukopenia exposed kittens. Question: In one of your online resources/publications regarding feline panleukopenia virus, it states that, Passive protection from serum transfusion has been shown in puppies exposed to canine parvovirus. Therefore, it may be that in exposed, unvaccinated kittens, 2 ml of serum from an immune cat given SC o
  2. A commercial hyperimmune serum, containing antibodies against feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and feline panleukopenia virus, is available for treatment of cats with feline upper respiratory tract disease (FURTD), but its efficacy has not been rigorously evaluated in scientific studies
  3. Feline panleukopenia (Feline Parvovirus, FPV) is a vaccine-preventable infectious disease. It is caused by feline parvovirus. It is environmentally resistant to many commonly used disinfectants. Vaccination or previous infections provide protection from reinfection

The disease is generally divided into two major genogroups: CPV-1 containing the classical feline panleukopenia virus (FPLV), and CPV-2 containing the canine parvovirus (CPV) which appeared in the 1970s. FPLV is known to infect all wild and domestic members of the felid (cat) family worldwide Blood samples drawn from each cat shall be individually tested for neutralizing antibody against feline panleukopenia virus to determine susceptibility. (i) A constant virus-carrying serum neutralization test in tissue culture using 100 to 300 TCID 50 of virus shall be used Feline Parvovirus (Panleukopenia) HI FPVHI: 1ml Serum (separated) Prices $34.00 Notes Ship chilled. Fever of Unknown Origin Diagnostic Plan Print Section. If case is chronic, virus isolation can be omitted if desired. If not previously performed a large animal chemistry panel may be warranted and serum (separated) or heparinized plasma would.

Feline Panleukopenia Vaccine shall be prepared from virus-bearing cell culture fluids. Only Master Seed Virus which has been established as pure, safe, and immunogenic shall be used for preparing the production seed virus for vaccine production. A constant virus-carrying serum neutralization test in tissue culture using 100 to 300 TCID 50. Feline (Parvovirus) Panleukopenia Titer. Sample: ½ ml serum, sample can be stored in refrigerator for up to two weeks before shipping. Price: $23/sample. Result: quantitative titers, with endpoint and interpretation of protection. Shipping: USPS priority mail (or similar), 2 day delivery. Overnight shipping is not necessary for this sample Prevalence of serum antibody titres against feline panleukopenia, herpesvirus and calicivirus infections in stray cats of Milan, Italy The aim of the study was to determine the seroprevalence of feline panleukopenia virus (FPV), feline herpesvirus type 1 (FHV-1) and feline calicivirus (FCV) in stray colony cats from Milan, Italy

Titer Testing Service. Canine Parvovirus-2 hemagglutination inhibition assay. Canine Distemper Virus serum virus neutralization assay. Feline Panleukopenia Virus hemagglutination inhibition assay. Other tests by request, including canine nomograph service. Our laboratory does NOT offer Canine Influenza, Leptospira, or Rabies titer testing 4. Vaccination may not equate to immunization; check serum titers to validate. 5. Long-term protection from canine distemper, hepatitis, parvovirus; feline panleukopenia. 6. Measure serum antibody (vaccine titers) instead annually or triennially. 7. Give thimerosal (mercury)-free rabies vaccines; and as late as allowed (20-24 weeks). 8 This correlation was established by comparing prechallenge serum-neutralization titers to the clinical response of felines challenged with feline panleucopenia virus. The serological and clinical response of felines inoculated with commercial vaccines or related viruses was also studied Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccinations against FPV among wild felid species have long been practiced in zoos worldwide. However, few studies have assessed the tiger immune response post-vaccination due to the absence of a serological diagnostic tool

Inactivated canine parvovirus (CPV) and inactivated feline panleukopenia virus (FPV) vaccines were evaluated in dogs. Maximal serologic response occurred within 1-2 weeks after vaccination. Antibody titers then declined rapidly to low levels that persisted at least 20 weeks. Immunity to CPV, defined as complete resistance to infection, was. Objective—To determine the proportion of cats entering a Florida animal shelter with serum antibody titers against feline panleukopenia virus (FPV), feline herpesvirus 1 (FHV1), and feline calicivirus (FCV) and to identify factors associated with seropositivity.. Design—Cross-sectional study.. Animals—347 cats admitted to a Florida animal shelter in serum and correlating these with protection or susceptibility. For certain diseases, such as feline panleukopenia, evaluation of antibody titers can be a valuable diagnostic indicator to determine when revac-cination may be needed.1,2 For other diseases, a serological response has not been identified that correlates with protection

Feline Panleukopenia - Generalized Conditions - Merck

Background. Feline parvovirus (FPV) is a common and potentially lethal infectious agent in cats. Objective. To assess the prognostic value of age, neuter status, serum concentrations of serum amyloid A (SAA), haptoglobin, cholesterol and total thyroxine (tT4), and the presence of systemic inflammatory response syndrome (SIRS) in cats with panleukopenia King DA, Croghan DL. Immunofluorescence of Feline panleukopenia virus in cell culture: determination of immunological status of felines by serum neutralization. Can J Comp Med Vet Sci 29: 85-89, 1965. PubMed: 1428107 that quantifying serum antibodies of either the queen or kittens before primary kitten vaccination may be useful in some situations to determine the optimum time for vaccina - FACT SHEET / Feline panleukopenia References 1 Pollock RVH and Postorino NC. Feline pan - leukopenia and other enteric viral diseases. In: Sherding RG (ed). The cat. Feline panleukopenia, also known as feline distemper is a very serious, often deadly, disease of cats. This requires serum from the blood that is sent off to a diagnostic laboratory. A polymerase chain reaction (PCR) test can also be performed in a diagnostic laboratory on feces or tissues from deceased cats Learn about panleukopenia, also called feline distemper, in cats, including risk factors, signs, symptoms, diagnosis, treatment, and prevention. Feline panleukopenia requires aggressive treatment if the cat is to survive, as this disease can kill cats in less than 24 hours. Learn about the veterinary topic of Overview of Feline Panleukopenia

Successful Treatment of Feline Panleukopenia: A Guideline

Feline Panleukopenia Virus - an overview ScienceDirect

RapidSTATUS™ Rapid4™. Lyme Aantibody / Canine Heartworm Antigen / Canine Ehrlichia sp Antibody / Canine Anaplasma sp Antibody. Sample Type - Blood/Serum. RapidSTATUS™ Titer Test™. Canine Distemper Virus Antibody / Canine Parvovirus Antibody / Canine Adenovirus type-2 Antibody. Sample Type - Blood/Serum Dawnsail fluorescent feline panleukopenia virus test kit is a fluorescent lateral flow strip used with Dawnsail's portable quantitative fluorescent reader. The feline panleukopenia virus test kit is able to detect feline panleukopenia virus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis Feline panleukopenia is a viral disease that usually causes a severe gastroenteritis. It is commonly called feline distemper, and is caused by a specific virus called a parvovirus. Without treatment, it has a very high mortality rate. Below is an overview on Feline Panleukopenia followed by in-depth information on the diagnosis and treatment of.

Feline panleukopenia - abcdcatsvets

  1. Feline panleukopenia is an extremely contagious and often fatal disease of cats. Learn more about the causes, symptoms and treatments of this scary disease. Serum is obtained from healthy cats or from groups of animals that have recovered from a disease, whereas hyperimmune serum comes from animals that had been repeatedly vaccinated. The.
  2. concentration and 4mmol/L serum potassium concentration. The relative risk of death for patients with serum albu
  3. . were diluted in medium 199 and incubated for one hour at room temp, with equal volumes of stock virus containing 100-300 TCID 50 per 0.1 ml. Aliquots of 0.2 ml. were added to secondary feline kidney cultures in Leighton tubes, which were maintained for four days at 37°C and then stained by the May.
(PDF) Prognostic Factors in Cats with Feline Panleukopenia

Feline panleukopenia by Dr Nadir Khosa. 1. D I R E C T E D B Y : D R . N A D I R K H O S A D V M , U V A S , L A H O R E Feline Panleukopenia. 2. Also known as; feline infectious enteritis feline parvoviral enteritis feline distemper feline ataxia cat plague. 3 FELINE PANLEUKOPENIA (FPL) is a highly contagious disease with a worldwide distribution in wild and domestic felids. It has been officially recorded in cat populations since the early 20th century but has likely existed for hundreds of years.1 Settings such as farms, animal shelters, pet shops, kennels, and research colonies are ideal for the spread and transmission of FPL of antibodies in serum and correlating these with protection or susceptibility. For certain diseases, such as feline panleukopenia, evaluation of antibody titers can be a valuable diagnostic indica-tor to determine when revaccination may be needed.1,2 For other diseases, a serological response has not been identified tha Feline panleukopenia titer (IgG & IgM) $24.00: Feline calicivirus antibody titer (IFA) Serum, CSF, swabs, EDTA blood, and effusions should be packed in a leak proof container with absorbent material. This package should be placed inside a second container on ice (gel packs). Samples should be clearly labeled Clearly, dogs and cats that have received appropriate puppy and kitten vaccine series do not need annual boosters thereafter, except for an additional booster a year later or serum vaccine titer measurements for the clinically significant viral disease, such as canine distemper and parvovirus for dogs, and feline panleukopenia virus for cats.

Feline Infectious Enteritis (Parvovirus, Panleukopenia

According to prior studies, between 25.0% and 92.8% of adult cats have antibodies against feline panleukopenia virus (FPV) and thus are likely protected against FPV infection. It is, however, unknown how healthy adult cats with different antibody titres react to FPV vaccination in the field VacciCheck. ®. Technical Information. Discover a world of superior pet protection, with VacciCheck. All the information you need on the only in-house titer test that tests for all 3 core vaccine diseases and compares favorably to Gold Standards Methods: Serum samples and kidneys were collected from 156 live and 26 cadaveric cats. (FCV), and feline panleukopenia virus (FPV) for use in feline viral vaccines such as feline viral rhinotracheitis, calicivirus, and panleukopenia (FVRCP) vaccine. Remnant protein

Feline panleukopenia caused by FPV may exist around the world. It has been considered one of the important infectious diseases of cats in Europe and North America. Serum amyloid A (SAA) is the main acute phase protein in cats and binds to plasma high-density lipoprotein (HDL). Clinical studies now focus on SAA types during the acute. Feline Panleukopenia Virus Antibody Test (IFA) Feline panleukopenia virus (FPV), also known as Feline infectious enteritis, Feline distemper, Feline ataxia, or cat plague, is a viral infection affecting cats, both domesticated and wild feline species. While often mistaken for feline distemper, the two conditions are not synonymous Feline panleukopenia is the oldest known viral disease of cats and is a well-known pathogen around the world. Carnivore protoparvovirus 1 is the base virus previously described, which contains two open reading frames (ORFs). ORF-L codes for two non-structural proteins, NS1 and NS2; ORF-R codes for two structural proteins, VP-1 and VP-2 (major.

All vaccines consisted of modified live panleukopenia virus. The booster injection was administered approximately 6 months after the initial parvovirus vaccination series was given. Fecal and serum specimens were collected immediately before and 2 weeks after administration of the booster vaccine for hemagglutination and hemagglutination. Q. Are serum antibody titres useful in determining vaccine immunity? A. Yes. They are especially useful for CDV, CPV-2 and CAV-1 in the dog, FPV in the cat, and rabies virus in the cat and dog. Rabies titers, however, are often not acceptable to exempt individual animals from mandated rabies boosters in spite of medical justification Feline Panleukopenia : Prevention. Feline Panleukopenia virus vaccine is typically administered in combination with feline herpesvirus-1 (FHV-1) and feline calicivirus (FCV). Initial vaccinations for nursing kittens generally begin at 8-9 weeks of age. Kittens should receive two of these vaccines, 3 to 4 weeks apart, with at least one dose after 12 weeks of age, because colostral antibodies.

Feline Panleukopenia - an overview ScienceDirect Topic

Panleukopenia (Feline Distemper, FPLV, Feline Parvo, FPV

  1. measuring serum neutralizing antibodies against CRP, using a standard microtitration technique by inhibition of cytopathic effects on microassay plates.11 The seroneutralization titer corresponds to the highest dilution of serum that neutralizes the viruses and is calculated using the Spearman and Kärber method.28 Titers are expressed in lOexp
  2. ation has been recommended as part of disease outbreak management in animal shelters
  3. Feline Panleukopenia Virus (FPL) IHC (IHC-1087) Fixed Tissue, Tissue Block or Unstained Slide : Feline: $32.00: More Feline Panleukopenia Virus (FPV) HI (SHI-0124) (0.5 ml) Serum (red top) or preservative-free tube Feline: $22.50: More Feline Panleukopenia Virus (FPV) PCR (MDL-3610
  4. feline respiratory diseases transmission. secretions of resp tract, eyes, mouth and nose. feline calici virus. less severe than rhino - 30% kittens die; URI symptoms, oral ulcers, limping syndrome - stiff, myalgia, limping. virulent calici virus. deadly systemic disease. chlamydophilia
  5. Prevalence of serum antibody titers against feline panleukopenia virus, feline herpesvirus 1, and feline calicivirus in cats entering a Florida Florida (49%). However, the specificity had decreased from 99% to animal shelter
  6. Titer testing is one method that is being studied in the hope of developing a safer approach to feline vaccination. Simple Feline Viral Diseases. The three simplest feline virues are feline panleukopenia (FPV), also known as feline distemper or feline parvovirus, calicivirus (FCV) and rhinotracheitis (feline herpes virus, or FHV)
  7. Biopsy (full) fixed tissue. $65.00 / $65.00. MVRDL. 2-3 working days for well fixed tissues with no special processing needed. Single site or tissue; masses are considered separate sites; multiple skin punch biopsies considered single site; allow more processing time for unfixed samples, bone, digits, whole organs

[PDF] Anti-feline panleukopenia virus serum neutralizing

Test Species Sample In State Price Lab Test Days or Turnaround Time Comments; More Equine Rhinopneumonitis SN: equin The high prevalence of human serum antibodies against adeno-associated virus type 2 (AAV) vectors represents a potential limitation for in vivo applications. Consequently, the development of AAV. Now, remember, when you're measuring the serum antibody level, it's not just the residual vaccine effects that you're measuring but also the exposure that that animal has had out in the real world. The reason for vaccinations is to effect protection upon exposure. Viruses like parvovirus and panleukopenia are everywhere. They're ubiquitous This kit is designed to determine cat serum IgG antibody titer to Feline Panleukopenia Virus (FPLV), Feline Herpes Virus (FHV) and Feline Calici Virus (FCV). The main purpose of this kit is to provide a useful tool for assessing immunity status of cats concerning these three pathogens. As such, it can either determine th

Video: 9 CFR § 113.304 - Feline Panleukopenia Vaccine. CFR US ..

Efficacy of passively transferred antibodies in cats with

Feline Panleukopenia Virus - Cat Health Guid

In croatia, It is impossible to obtain Feliserin (anti-fpv) serum used to treat panleukopenia in cats . We are engaged in providing care, castration and sterilization of feral cats and we have a constant need for it . Currently the drug is purchased from Slovenia at a price of 20 euros per dose Napomena: sljedeći tekst nisu pisali veterinari, već volonteri koji imaju iskustva u borbi s ovom bolešću. Namijenjen je svima koji spašavaju mace s ulica, kako bi edukacijom minimizirali mogućnost zaraze. Panleukopenija je nažalost neminovna bolest, koju je priroda dizajnirala kako bi prorijedila mačju populaciju koja se nekontrolirano razmnožava

Feline panleukopenia virus belongs to which family. A parvovirus belonging to the viral family Parvoviridae. only one serotype is recognized. Can give hyperimmune serum prophylactically. Treatment for panleukopenia. Withold food and milk Give fluids and antibiotics Disinfection of premises with 6% hypochlorite RapidSTATUS™ Rapid4™. Analyte - Lyme Aantibody / Canine Heartworm Antigen / Canine Ehrlichia sp Antibody / Canine Anaplasma sp Antibody. Sample Type - Blood/Serum. RapidSTATUS™ TiterTest™. Analyte - Canine Distemper Virus Antibody / Canine Parvovirus Antibody / Canine Adenovirus type-2 Antibody. Sample Type - Blood/Serum

Carnivore protoparvovirus 1 - Wikipedi

Dawnsail fluorescent feline panleukopenia virus test kit is a fluorescent lateral flow strip used with Dawnsail's portable quantitative fluorescent reader.. The feline panleukopenia virus test kit is able to detect feline panleukopenia virus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis Tissue: 1-10g, serum: 1-2ml, whole blood: 5-10ml, Collection Information GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date Vaccines for Feline Panleukopenia Virus. Inactivated vaccines. Inactivated FPV vaccines have been developed at Creative Biolabs due to their good safety. Such vaccine can be administered by injection and provides solid immunity to the disease. A single dose of inactivated FPV vaccine can rapidly induce a good antibody response in naive cats Leukopenia is so often caused from a decrease in neutrophils that some people use the terms leukopenia and neutropenia interchangeably. Another common type of leukopenia is.

DiGangi BA et al. Prevalence of serum antibody titers against feline panleukopenia virus, feline herpesvirus 1, and feline calicivirus in cats entering a Florida animal shelter. J Am Vet Med Assoc. 2012 Nov 15;241(10):1320-1325. Protect all cats - don't try to predict who may or may not have previously been vaccinated Sera were tested for antibodies against Feline Panleukopenia Virus (FPV) by hemagglutination inhibition test and serum neutralisation assay in two independent diagnostic laboratories. Results There was a good correlation between the results obtained in different laboratories and with different methods Feline parvovirus (FP), also known as feline panleukopenia virus (FPV), is responsible for causing disease in cats of all ages. This disease varies in its symptoms with ages of cats and is known as feline panleukopenia (1). The virus's genetic material is single-stranded deoxyribonucleic acid (DNA) Infection by Feline parvovirus was thought only to occur in cats (Feline panleukopenia virus, FPLV) or raccoons until the mid-1940s, when a similar disease with a mortality of up to 80% was observed in infected mink kits in Canada ().The disease caused by the mink agent, named Mink enteritis virus (MEV), was thereafter observed throughout many regions of the world () Blood samples drawn from each cat shall be individually tested for neutralizing antibody against feline panleukopenia virus to determine susceptibility. (i) A constant virus-carrying serum neutralization test in tissue culture using 100 to 300 TCID50 of virus shall be used

China Anaplasma - Ehrlichia Antibody Combo Test(PDF) Detection of Protective Antibody Titers againstVeterinary laboratory reagent - MYKODERMOASSAY DTMOne Step CRP Semi-Quantitative Rapid Test Device (Whole

The tamaVet® Canine Distemper Virus (CDV) Test is intended for the rapid detection of canine distemper antigens in serum, urine, faeces or conjunctival swabs in dogs. The disease is caused by canine distemper virus, which belongs to the Paramyxoviridae family. Transmission tends to be intrauterine or as a result of direct contact This example illustrates the production of a panleukopenia vaccine using ferret tissue. 50,000,000 cells of ferret embryo tissue and 50,000,000 FAID 50 of virus, suspended in 2 liters of Minimum Essential Medium (Eagle's), with non-essential amino acids and glutamine, 10% fetal bovine serum, 100 units of penicillin and 100 mg. of streptomicin. Feline distemper, also known as feline panleukopenia, is a contagious disease that's caused by a single-stranded DNA virus.Cats become infected with the virus through direct contact with contaminated sources. Kittens are more susceptible to the virus and once infected they're capable of shedding the virus in the environment even after complete recovery 93,3. 99,9. The tamaVet® Parvovirus Test is intended for the rapid detection of specific canine and feline parvovirus antigens in faecal samples taken from dogs and cats. Both canine parvovirus (CPV2, 2a, 2b, 2c) and feline panleukopenia virus (FPV) belong to the Parvoviridae family. Transmission is usually oral or intrauterine

Current Biomarkers | BenthamScienceCanine Heartworm Test Kit - CHW Ag Test - ELisa Test Kit

1 mL serum. 1-2 days. 6723. Heartworm Panel-Canine Canine heartworm antigen by ELISA, microfilariae by filter. 1 mL serum, 2mL LTT. 1-2 days. 6271. Lyme Quant C6 ® Antibody by ELISA. 1 mL serum. 2-4 days. 67246. Spec fPL ® Test-Feline. 1 mL serum (fasted preferred) 1-2 days. 62493. Spec cPL ® Test-Canine. 1 mL serum (fasted. Table 2. Serum chemistry values for snow leopards infected with feline panleukopenia virus and canine distemper virus. Serum chemistry Sex and date Female Male 11/11/88 11/13/88 11/27/88 12/14/88 Normal8 X (SD) Sodium (mEq/L) 142 148 149 142 152 (2.9) Potassium (mEq/L) 3.3 3.4 3.7 4.7 4.4(0.4) Chloride (mEq/L) 120 116 121 114 120 (3.5 ELISA test kits for in-house or foal-side testing can use either serum or whole blood as the sample and take ~10 min. Most are semiquantitative, with color changes corresponding to IgG concentrations of <400 mg/dL, 400-800 mg/dL, or >800 mg/dL. One quantitative handheld colorimetric immunoassay is currently available Serum (2 mL) is requested for these tests and this testing can be done in combination with other tests sent to the laboratory, e.g. thyroid function tests. Feline Panleukopenia Virus (FPV) HI (80035) We recommend revaccination for cats with an FHV-1 titer below 16, an FCV titer below 32, and an FPV titer below 40